Clonase manual

THe manual (invitrogen) says to use the new LR clonase II plus, which is more expensive than the LR clonase II. I was wondering if anyone have tried doing multisite Gateway with the normal LR. Gateway Clonase Manual The Donor Vector box, the Gateway® BP Clonase®II Enzyme Mix and Reagents box, and the M13 Sequencing Primers box (Boxes 1–4) contain the following items. Store the Gateway® BP Clonase®II enzyme mix at −30°C to −10°C for up to 6 months. For long-term storage, store at −85°C to −68°C. LR Clonase II Plus Enzyme Mix. Single mix of enzyme and reaction buffer. Highest cloning efficiency for single and multiple fragment cloning. Optimized for difficult cloning reactions. Works with MultiSite Gateway Pro Technology. Easy to use single mix format ensures enzyme stability. Convenient 10 µl reaction set up. Very good.

5X LR ClonaseŽ Reaction Buffer 4 µl TE buffer, pH to 16 µl 2. Remove LR Clonase enzyme mix from °C and thaw on ice for about 2 minutes. Vortex the LR ClonaseŽ enzyme mix briefly twice (2 seconds each time). 3. To each sample (Step 1, above), add 4 µl of LR Clonase enzyme mix to the reaction and mix well by vortexing briefly twice. The Donor Vector box, the Gateway® BP Clonase® II Enzyme Mix and Reagents box, and the M13 Sequencing Primers box (Boxes 1–4) contain the following items. Store the Gateway® BP Clonase® II enzyme mix at −30°C to −10°C for up to 6 months. LR Clonase™ II enzyme mix is supplied as a 5X solution. If you wish to scale the reaction volume, make sure the LR Clonase™ II enzyme mix is at a final concentration of 1X. For a positive control, use ng (2 μL) of pENTR™-gus. 1. Add the following components to a mL microcentrifuge tube at room temperature and mix.

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